DNA

Part:BBa_K5008000:Design

Designed by: Chengyao Yin   Group: iGEM23_Squirrel-Shanghai   (2023-10-10)


CsgA_MBP


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 371
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 371
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 371
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 371
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

CsgA (BBa_K4161010) is widely used in the development of living biological materials. It participates in the formation of E. coli flagella, and E. coli has many flagella. Therefore, after CsgA is fused and expressed with other proteins, E. coli can be given the function of broadly presenting proteins. For example, the cases in Figure 2 and corresponding references demonstrate the fusion of CsgA and the mineralizing short peptide A7 to confer biofilm mineralization ability.


Source

atgaaacttttaaaagtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgctgcagttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagtacGGCGGCAGCGGCAGCctgaccccgctgaccaccagccatctgcgcagc

References